Characterization of the RNase R association with ribosomes.
BMC Microbiol
; 14: 34, 2014 Feb 11.
Article
em En
| MEDLINE
| ID: mdl-24517631
ABSTRACT
BACKGROUND:
In this study we employed the TAP tag purification method coupled with mass spectrometry analysis to identify proteins that co-purify with Escherichia coli RNase R during exponential growth and after temperature downshift.RESULTS:
Our initial results suggested that RNase R can interact with bacterial ribosomes. We subsequently confirmed this result using sucrose gradient ribosome profiling joined with western blot analysis. We found that RNase R co-migrates with the single 30S ribosomal subunits. Independent data involving RNase R in the rRNA quality control process allowed us to hypothesize that the RNase R connection with ribosomes has an important physiological role.CONCLUSIONS:
This study leads us to conclude that RNase R can interact with ribosomal proteins and that this interaction may be a result of this enzyme involvement in the ribosome quality control.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ribossomos
/
Proteínas de Escherichia coli
/
Escherichia coli
/
Exorribonucleases
Tipo de estudo:
Risk_factors_studies
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article