Expanding the potential of G-quadruplex structures: formation of a heterochiral TBA analogue.
Chembiochem
; 15(5): 652-5, 2014 Mar 21.
Article
em En
| MEDLINE
| ID: mdl-24520055
ABSTRACT
In order to expand the potential applications of G-quadruplex structures, we explored the ability of heterochiral oligodeoxynucleotides based on the thrombin-binding aptamer (TBA) sequence to fold into similar complexes, with particular focus on their resistance in biological environments. A combination of CD and NMR techniques was used. Similarly to TBA, the ODN ggTTggtgtggTTgg (lower case letters indicate L residues) is able to fold into a chair-like antiparallel G-quadruplex structure, but has a slightly higher thermal stability. The discovery that heterochiral ODNs are able to form stable G-quadruplex structures opens up new possibilities for their development in several fields, as aptamers, sensors and, as recently shown, as catalysts for enantioselective reactions.
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Base de dados:
MEDLINE
Assunto principal:
Oligodesoxirribonucleotídeos
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Aptâmeros de Nucleotídeos
/
Quadruplex G
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article