α-catenin cytomechanics--role in cadherin-dependent adhesion and mechanotransduction.
J Cell Sci
; 127(Pt 8): 1779-91, 2014 Apr 15.
Article
em En
| MEDLINE
| ID: mdl-24522187
ABSTRACT
The findings presented here demonstrate the role of α-catenin in cadherin-based adhesion and mechanotransduction in different mechanical contexts. Bead-twisting measurements in conjunction with imaging, and the use of different cell lines and α-catenin mutants reveal that the acute local mechanical manipulation of cadherin bonds triggers vinculin and actin recruitment to cadherin adhesions in an actin- and α-catenin-dependent manner. The modest effect of α-catenin on the two-dimensional binding affinities of cell surface cadherins further suggests that force-activated adhesion strengthening is due to enhanced cadherin-cytoskeletal interactions rather than to α-catenin-dependent affinity modulation. Complementary investigations of cadherin-based rigidity sensing also suggest that, although α-catenin alters traction force generation, it is not the sole regulator of cell contractility on compliant cadherin-coated substrata.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Caderinas
/
Adesão Celular
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Mecanotransdução Celular
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Alfa Catenina
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article