Oxidation-responsive OEGylated poly-L-cysteine and solution properties studies.
Biomacromolecules
; 15(3): 1055-61, 2014 Mar 10.
Article
em En
| MEDLINE
| ID: mdl-24527760
ABSTRACT
The oxidation-responsive behaviors of OEGylated poly-L-cysteine homopolypeptides, that is, poly(L-EG(x)MA-C)n, were investigated. These poly-L-cysteine derivatives adopted mixed conformation in water, in which the ß-sheet accounted for a significant proportion. Upon oxidation, the thioethers in polypeptide side chains were converted to polar sulfone groups, which triggered the secondary structure transition from ß-sheet preferred conformation to random coil. Accordingly, the increase of side-chain polarity together with conformation changes increased samples' water solubility and cloud point temperature. Using mPEG45-NH2 as macroinitiator, we synthesized PEG45-b-poly(L-EG2MA-C)22 diblock copolymer via ring-opening polymerization (ROP) of L-EG2MA-C N-carboxyanhydride (NCA). The PEG45-b-poly(L-EG2MA-C)22 was able to self-assemble into spherical micelles in aqueous solution, and the micelles could undergo an oxidation-triggered disassembly due to the oxidation-responsive thioethers. Such a new class of oxidation-responsive polypeptides might provide a promising platform to construct inflammation targeting drug delivery systems.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Polietilenoglicóis
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Sistemas de Liberação de Medicamentos
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Inflamação
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article