Probing the ubiquinol-binding site of recombinant Sauromatum guttatum alternative oxidase expressed in E. coli membranes through site-directed mutagenesis.

Young, Luke; May, Benjamin; Pendlebury-Watt, Alice; Shearman, Julia; Elliott, Catherine; Albury, Mary S; Shiba, Tomoo; Inaoka, Daniel Ken; Harada, Shigeharu; Kita, Kiyoshi; Moore, Anthony L

Young, Luke; May, Benjamin; Pendlebury-Watt, Alice; Shearman, Julia; Elliott, Catherine; Albury, Mary S; Shiba, Tomoo; Inaoka, Daniel Ken; Harada, Shigeharu; Kita, Kiyoshi; Moore, Anthony L.

Afiliação

Young L; Biochemistry and Molecular Sciences, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG, UK.
May B; Biochemistry and Molecular Sciences, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG, UK.
Pendlebury-Watt A; Biochemistry and Molecular Sciences, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG, UK.
Shearman J; Biochemistry and Molecular Sciences, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG, UK.
Elliott C; Biochemistry and Molecular Sciences, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG, UK.
Albury MS; Biochemistry and Molecular Sciences, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG, UK.
Shiba T; Department of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Kyoto 606-8585, Japan.
Inaoka DK; Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
Harada S; Department of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Kyoto 606-8585, Japan.
Kita K; Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
Moore AL; Biochemistry and Molecular Sciences, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG, UK. Electronic address: a.l.moore@sussex.ac.uk.

Biochim Biophys Acta ; 1837(7): 1219-25, 2014 Jul.

Article em En | MEDLINE | ID: mdl-24530866

Assuntos

Araceae/enzimologia; Proteínas Mitocondriais/metabolismo; Mutação de Sentido Incorreto; Oxirredutases/metabolismo; Proteínas de Plantas/metabolismo; Ubiquinona/análogos & derivados; Sequência de Aminoácidos; Sítios de Ligação; Membrana Celular/metabolismo; Escherichia coli/genética; Escherichia coli/metabolismo; Proteínas Mitocondriais/antagonistas & inibidores; Proteínas Mitocondriais/química; Proteínas Mitocondriais/genética; Dados de Sequência Molecular; Mutagênese Sítio-Dirigida; Oxirredutases/antagonistas & inibidores; Oxirredutases/química; Oxirredutases/genética; Proteínas de Plantas/antagonistas & inibidores; Proteínas de Plantas/química; Proteínas de Plantas/genética; Proteínas Recombinantes/química; Proteínas Recombinantes/genética; Proteínas Recombinantes/metabolismo; Terpenos/farmacologia; Ubiquinona/metabolismo

Palavras-chave

AOX inhibitors; Alternative oxidase; Escherichia coli membrane; Oxygen affinity; Quinol oxidase; Site-directed mutagenesis; Structurefunction relation

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Oxirredutases / Proteínas de Plantas / Ubiquinona / Mutação de Sentido Incorreto / Araceae / Proteínas Mitocondriais Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2014 Tipo de documento: Article

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