Ancient whole genome enrichment using baits built from modern DNA.
Mol Biol Evol
; 31(5): 1292-4, 2014 May.
Article
em En
| MEDLINE
| ID: mdl-24531081
ABSTRACT
We report metrics from complete genome capture of nuclear DNA from extinct mammoths using biotinylated RNAs transcribed from an Asian elephant DNA extract. Enrichment of the nuclear genome ranged from 1.06- to 18.65-fold, to an apparent maximum threshold of â¼80% on-target. This projects an order of magnitude less costly complete genome sequencing from long-dead organisms, even when a reference genome is unavailable for bait design.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Genoma
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Análise de Sequência de DNA
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Genômica
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Mamutes
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article