In vitro assay of pre-mRNA splicing in mammalian nuclear extract.
Methods Mol Biol
; 1126: 151-60, 2014.
Article
em En
| MEDLINE
| ID: mdl-24549662
ABSTRACT
The in vitro splicing assay is a valuable technique that can be used to study the mechanism and machinery involved in the splicing process. The ability to investigate various aspects of splicing and alternative splicing appears to be endless due to the flexibility of this assay. Here, we describe the tools and techniques necessary to carry out an in vitro splicing assay. Through the use of radiolabeled pre-mRNA and crude nuclear extract, spliced mRNAs can be purified and visualized by autoradiography for downstream analysis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Precursores de RNA
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Processamento Alternativo
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Biologia Molecular
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article