Kinetic analysis of in vitro pre-mRNA splicing in HeLa nuclear extract.
Methods Mol Biol
; 1126: 161-8, 2014.
Article
em En
| MEDLINE
| ID: mdl-24549663
ABSTRACT
Kinetic analysis of in vitro splicing is a valuable technique for understanding splicing regulation. It allows the determination of specific contributions from functional elements for the efficient removal of introns. This chapter will describe the rationale and approach employed to use kinetic analysis to evaluate an in vitro splicing reaction using radiolabeled pre-mRNA incubated in splicing-competent HeLa nuclear extract (NE).
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Extratos Celulares
/
Processamento Alternativo
/
Biologia Molecular
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article