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Production and characterization of alpha-amylase from mango kernel by Fusarium solani NAIMCC-F-02956 using submerged fermentation.
Kumar, Devendra; Yadav, Kaushlesh K; Muthukumar, M; Garg, Neelima.
Afiliação
  • Kumar D; Division of Post Harvest Management, Central Institute for Subtropical Horticulture, Lucknow - 227 107, India.
  • Yadav KK; Division of Post Harvest Management, Central Institute for Subtropical Horticulture, Lucknow - 227 107, India.
  • Muthukumar M; Division of Crop Improvement and Biotechnology, Central Institute for Subtropical Horticulture, Lucknow - 227 107, India.
  • Garg N; Division of Post Harvest Management, Central Institute for Subtropical Horticulture, Lucknow - 227 107, India.
J Environ Biol ; 34(6): 1053-8, 2013 Nov.
Article em En | MEDLINE | ID: mdl-24555336
Microbial production of enzymes using low valued agro industrial wastes is gaining importance globally. Mango is one of the major fruit processed into a variety of products. During processing 40-50% of solid waste is generated in form of peel and stones. After decortications of mango stone, kernel is obtained which is a rich source of starch (upto 60%). It was utilized as a substrate for alpha-amylase production using Fusarium soloni. Maximum alpha-amylase production (0.889 U g(-1)) was recorded using a substrate concentration of 5% (w/v), pH-4 and temperature 30 degrees C on 9th day of incubation. Supplementation of production medium with micronutrients viz., Ca2+, Fe2+ or Mg2+ improved the enzyme production while, Zn2+, B3+ or Mn2+ ions exhibited inhibitory effect. The extracellular protein was precipitated by ammonium sulphate up to 70% saturation, dialyzed and purified (27.84 fold) by gel-exclusion (Sephadex G-75) chromatography. Protein profiling on 12% SDS-PAGE revealed three bands corresponding to 26, 27 and 30 kDa molecular sizes. The optimum amylase activity was achieved at pH 5.0 at 40 degrees C. The Michaelis constant (KM), Vmax and activation energy (-Ea) were found to be 3.7 mg ml(-1), 0.24 U mg(-1) and 42.39 kJ mole(-1), respectively.
Assuntos
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Base de dados: MEDLINE Assunto principal: Mangifera / Alfa-Amilases / Fusarium Tipo de estudo: Evaluation_studies Idioma: En Ano de publicação: 2013 Tipo de documento: Article
Buscar no Google
Base de dados: MEDLINE Assunto principal: Mangifera / Alfa-Amilases / Fusarium Tipo de estudo: Evaluation_studies Idioma: En Ano de publicação: 2013 Tipo de documento: Article