Microfluidic, marker-free isolation of circulating tumor cells from blood samples.
Nat Protoc
; 9(3): 694-710, 2014 Mar.
Article
em En
| MEDLINE
| ID: mdl-24577360
ABSTRACT
The ability to isolate and analyze rare circulating tumor cells (CTCs) has the potential to further our understanding of cancer metastasis and enhance the care of cancer patients. In this protocol, we describe the procedure for isolating rare CTCs from blood samples by using tumor antigen-independent microfluidic CTC-iChip technology. The CTC-iChip uses deterministic lateral displacement, inertial focusing and magnetophoresis to sort up to 107 cells/s. By using two-stage magnetophoresis and depletion antibodies against leukocytes, we achieve 3.8-log depletion of white blood cells and a 97% yield of rare cells with a sample processing rate of 8 ml of whole blood/h. The CTC-iChip is compatible with standard cytopathological and RNA-based characterization methods. This protocol describes device production, assembly, blood sample preparation, system setup and the CTC isolation process. Sorting 8 ml of blood sample requires 2 h including setup time, and chip production requires 2-5 d.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Separação Celular
/
Técnicas Analíticas Microfluídicas
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Células Neoplásicas Circulantes
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article