Imaging dynamic molecular signaling by the Cdc42 GTPase within the developing CNS.
PLoS One
; 9(2): e88870, 2014.
Article
em En
| MEDLINE
| ID: mdl-24586421
ABSTRACT
Protein interactions underlie the complexity of neuronal function. Potential interactions between specific proteins in the brain are predicted from assays based on genetic interaction and/or biochemistry. Genetic interaction reveals endogenous, but not necessarily direct, interactions between the proteins. Biochemistry-based assays, on the other hand, demonstrate direct interactions between proteins, but often outside their native environment or without a subcellular context. We aimed to achieve the best of both approaches by visualizing protein interaction directly within the brain of a live animal. Here, we show a proof-of-principle experiment in which the Cdc42 GTPase associates with its alleged partner WASp within neurons during the time and space that coincide with the newly developing CNS.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sistema Nervoso Central
/
Proteína cdc42 de Ligação ao GTP
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Imagem Molecular
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Simulação de Dinâmica Molecular
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article