Early diagnosis of orthopedic implant failure using macromolecular imaging agents.
Pharm Res
; 31(8): 2086-94, 2014 Aug.
Article
em En
| MEDLINE
| ID: mdl-24590878
ABSTRACT
PURPOSE:
To develop and evaluate diagnostic tools for early detection of wear particle-induced orthopaedic implant loosening.METHODS:
N-(2-Hydroxypropyl)methacrylamide (HPMA) copolymer was tagged with a near infrared dye and used to detect the inflammation induced by polymethylmethacrylate (PMMA) particles in a murine peri-implant osteolysis model. It was established by inserting an implant into the distal femur and challenging with routine PMMA particles infusion. The osteolysis was evaluated by micro-CT and histological analysis at different time points.RESULTS:
Significant peri-implant osteolysis was found 3-month post PMMA particle challenge by micro-CT and histological analysis. At 1-month post challenge, when there was no significant peri-implant bone loss, the HPMA copolymer-near infrared dye conjugate was found to specifically target the femur with PMMA particles deposition, but not the contralateral control femur with phosphate buffered saline (PBS) infusion.CONCLUSION:
The results from this study demonstrate the feasibility of utilizing the macromolecular diagnostic agent to detect particle-induced peri-implant inflammation prior to the development of detectable osteolysis. Recognition of this early pathological event would provide the window of opportunity for prevention of peri-implant osteolysis and subsequent orthopaedic implant failure.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Próteses e Implantes
/
Falha de Prótese
/
Fluoresceína
/
Fêmur
/
Metacrilatos
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article