Inhibiting mast cell degranulation by HO-1 affects dendritic cell maturation in vitro.
Inflamm Res
; 63(7): 527-37, 2014 Jul.
Article
em En
| MEDLINE
| ID: mdl-24604352
ABSTRACT
OBJECTIVE AND DESIGN:
Mast cell (MC) degranulation can break peripheral immune tolerance. However, its mechanism remains unclear. Our goal was to study the stabilization of MC membranes by heme oxygenase-1 (HO-1) in order to influence dendritic cell (DC) function.MATERIAL:
Mast cells and dendritic cells were prepared from 8-week-old to 10-week-old C57BL/6 mice; spleen mononuclear cells (SMCs) were prepared from 8-week-old to 10-week-old C57BL/6 and Balb/c mice. TREATMENT Mast cells were pretreated with PBS, DMSO, Hemin (50 µl/ml), and Znpp (50 µl/ml) for 8 h.METHOD:
Real-time PCR and western-blot tested the HO-1 of MC mRNA and protein. The co-stimulatory molecules of DCs (CD80, CD86, CD40) were measured by flow cytometry, and levels of TNF-α, IL-6, and IFN-γ were measured by ELISA. We set up a one-way mixed lymphocyte reaction (MLR) model to test the proliferation of SMCs after MC/DC interaction. *P < 0.05 (t test) was taken as the level of statistical significance.RESULT:
MCs pretreated with hemin induced HO-1 mRNA and protein expression, then interacted with DCs; expression of the co-stimulatory molecules was attenuated. The TNF-α, IL-6, and IFN-γ levels in the co-culture system were decreased. These DCs couldn't stimulate the proliferation of SMCs.CONCLUSION:
Inhibiting MC degranulation by HO-1 restrained DC maturation and attenuated the proliferation of SMCs.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Células Dendríticas
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Leucócitos Mononucleares
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Heme Oxigenase-1
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Mastócitos
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Proteínas de Membrana
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article