Improving sensitivity in microchip electrophoresis coupled to ESI-MS/MS on the example of a cardiac drug mixture.
Electrophoresis
; 35(12-13): 1880-6, 2014 Jul.
Article
em En
| MEDLINE
| ID: mdl-24610686
ABSTRACT
A comprehensive study for a sensitivity optimization in MCE with mass spectrometric detection is presented. As a text mixture, we chose a mixture of the cardiac drugs propranolol, bisoprolol, lidocaine, procaine and studied the effect of different chip layouts and experimental parameters with the aim of achieving both high sensitivity in MS detection and adequate chip electrophoretic separation. An important aspect was a comparison of microfluidic layouts containing various sheath-flow channels with that avoiding sheath-flow junctions on-chip. We utilized glass chips with monolithically integrated nanospray emitter tips coupled dead volume-free to an IT mass spectrometer running in fragmentation mode (MS(n) ). With this setup, detection limits down to 0.6 ng/mL for the model compound propranolol were achieved.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Fármacos Cardiovasculares
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Espectrometria de Massas por Ionização por Electrospray
/
Eletroforese em Microchip
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article