Toward the generation of an aminonaphthalene trisulfonate labeled N-glycan database for capillary gel electrophoresis analysis of carbohydrates.

ABSTRACT

There is an increasing trend to develop therapeutic glycoproteins, mostly antibodies that require high resolution bioanalytical tools to address the challenging aspects of comprehensive carbohydrate characterization. In this paper we introduce an initial version of a glucose unit database for 8-aminonaphthalene-1,3,6-trisulfonic acid-labeled glycans. At this stage we mainly focused on therapeutic IgG derived glycans of core fucosylated biantennary structures with and without sialic acid residues, as well as high mannose structures. Currently 25 oligosaccharides represent this first set of the database that shows the abbreviated names of the individual sugar structures with their graphic representation, precise molecular mass and glucose unit (GU) values with corresponding SDs. The database will provide a quick glycan analysis tool for preliminary data interpretation of rapid (around 200 s) CGE-LED-induced fluorescence (CGE-LEDIF) based glycan profiling runs.