RECK overexpression reduces invasive ability in ameloblastoma cells.
J Oral Pathol Med
; 43(8): 613-8, 2014 Sep.
Article
em En
| MEDLINE
| ID: mdl-24646032
ABSTRACT
BACKGROUND:
Ameloblastoma is a frequent odontogenic neoplasm characterized by local invasiveness and high risk of recurrence. Reversion-inducing cysteine-rich protein with Kazal motifs (RECK) is a tumor suppressor that inhibits metastasis and angiogenesis. The aim of this study was to investigate effects of RECK overexpression on invasive potential in ameloblastoma cells.METHODS:
Lentiviral vectors containing human RECK gene were created and subsequently stably transfected into immortalized ameloblastoma cell line hTERT(+) -AM. Functional characteristics of hTERT(+) -AM cells with stable RECK overexpression included proliferation, migration, invasion, and regulation of matrix metalloproteinases (MMP)-2, MMP-9 measured by zymography or commercially available assays.RESULTS:
The stable and higher expression of RECK mRNA and protein (P < 0.01) was detected in RECK-transfected hTERT(+) -AM cells. RECK overexpression caused a decrease in migration and invasion (P < 0.01) for hTERT(+) -AM cells and a decrease in activity of MMP-2, MMP-9 (P < 0.01). Proliferation was not affected by RECK overexpression (P > 0.05).CONCLUSIONS:
Overexpression of RECK gene significantly inhibited cell invasive ability of hTERT(+) -AM cells, suggesting RECK may be a new target for ameloblastoma treatment.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ameloblastoma
/
Proteínas Ligadas por GPI
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article