Effects of calcitonin gene-related peptide on synaptic acetylcholine receptor-channels in rat muscle fibres.
Proc R Soc Lond B Biol Sci
; 234(1276): 333-42, 1988 Aug 23.
Article
em En
| MEDLINE
| ID: mdl-2464831
ABSTRACT
Cultured myotubes and freshly dissociated muscle fibres from adult rats were exposed to calcitonin gene-related peptide (CGRP) and studied by patch-clamp recording during the peptide-induced maximal accumulation of cellular cyclic AMP (cAMP). Acetylcholine receptor- (AChR-) channel properties in myotubes were not modified by the presence of CGRP (10(-7) M). The peptide, applied to the non-patched membrane, significantly increased the variance of the AChR-channel amplitude distribution at the synaptic region of muscle fibres, and three classes of AChR-channels were resolved immediately after peptide application. AChR-channels at extrasynaptic regions of fibres from denervated muscles were unaffected by CGRP. It is suggested that CGRP may regulate the synaptic AChR-channel conductance through second messenger systems.
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Base de dados:
MEDLINE
Assunto principal:
Sinapses
/
Neuropeptídeos
/
Calcitonina
/
Receptores Colinérgicos
/
AMP Cíclico
/
Músculos
Limite:
Animals
Idioma:
En
Ano de publicação:
1988
Tipo de documento:
Article