Importance of promoter methylation of GATA4 and TP53 genes in endometrioid carcinoma of endometrium.
Clin Chem Lab Med
; 52(8): 1229-34, 2014 Aug.
Article
em En
| MEDLINE
| ID: mdl-24651021
ABSTRACT
BACKGROUND:
Epigenetic changes are considered to be a frequent event during tumor development. Various methylation changes have been identified and show promise as potential cancer biomarkers. The aim of this study was to investigate promoter methylation of GATA4 and TP53 genes in endometrioid carcinoma of endometrium.METHODS:
To search for promoter methylation of GATA4 and TP53 genes we used methylation-specific PCR (MSP) to compare the methylation status of 54 patients with endometrioid carcinoma of endometrium and 18 patients with normal endometrial tissue.RESULTS:
In our study MSP revealed GATA4 promoter methylation in 44 of 54 in the carcinoma group (81.5%), and in none of the control group. No methylation was observed in TP53 gene.CONCLUSIONS:
In conclusion, our study showed that there is significantly higher methylation in GATA4 gene in the endometrial cancer group compared with samples of non-neoplastic endometrium. The finding suggests the importance of hypermethylation of this gene in endometrial carcinogenesis and could have implications for future diagnostic and therapeutic strategies for endometrial cancer based on epigenetic changes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Genes p53
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Neoplasias do Endométrio
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Carcinoma Endometrioide
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Metilação de DNA
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Fator de Transcrição GATA4
Tipo de estudo:
Observational_studies
/
Prognostic_studies
Limite:
Adult
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Aged
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Aged80
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Female
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Humans
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Middle aged
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article