Long-term stability in biomass and production of terpene indole alkaloids by hairy root culture of Rauvolfia serpentina and cost approximation to endorse commercial realism.
Biotechnol Lett
; 36(7): 1523-8, 2014 Jul.
Article
em En
| MEDLINE
| ID: mdl-24658738
ABSTRACT
The effect of 6 years of cultivation and use of table-sugar (TS) on the biomass/terpene alkaloid productivities and rol gene expression were studied in a hairy root (HR) clone of Rauvolfia serpentina. The media cost could be reduced >94 % by replacing sucrose (SUC) with TSan unexplored avenue for HR cultivation. The overall productivities increased over long-term cultivation with sugar proving superior to SUC for biomass (24.4 ± 2.11 g/l DW after 40 days to 17.31 % higher) and reserpine (0.094 ± 0.008 % DW after 60 days to 193.8 % more) production. The latter however revealed comparatively better yields concerning ajmaline (0.507 ± 0.048 % DW after 60 days to 61.98 % higher) and yohimbine (0.628 ± 0.062 % DW after 60 days to 38.32 % higher), respectively. PCR amplification of rol genes confirmed long-term expression stability.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Rauwolfia
/
Terpenos
/
Biotecnologia
/
Raízes de Plantas
/
Alcaloides Indólicos
/
Alcaloides de Triptamina e Secologanina
Tipo de estudo:
Health_economic_evaluation
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article