Detection of enterovirus genome sequence from diarrheal feces of goat.
Virus Genes
; 48(3): 550-2, 2014 Jun.
Article
em En
| MEDLINE
| ID: mdl-24691818
ABSTRACT
Goat diarrheal feces were subjected to metagenome analysis by the next-generation sequencing. Nucleotide sequences with homology to enteroviruses were obtained. Primers for RT-PCR were designed based on the nucleotide sequence of these sequences at the 5'-untranslated region, and we determined 563 bp nucleotide sequences that showed homology to bovine-like and ovine enteroviruses (77-87 %). We named the virus detected in this study goat enterovirus G1 (GEV-G1). In the phylogenetic analysis, GEV-G1 belonged to a cluster containing ovine enteroviruses. To our knowledge, this is the first report on nucleotide sequences of an enterovirus infecting Japanese goats.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Doenças das Cabras
/
Genoma Viral
/
Enterovirus
/
Diarreia
/
Fezes
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article