Lithium carbonate teratogenic effects in chick cardiomyocyte micromass system and mouse embryonic stem cell derived cardiomyocyte--possible protective role of myo-inositol.
Reprod Toxicol
; 46: 106-14, 2014 Jul.
Article
em En
| MEDLINE
| ID: mdl-24703859
ABSTRACT
The drug lithium carbonate (Li2CO3) use during pregnancy increases the possibility of cardiovascular anomalies. The earlier studies confirm its phosphatidylinositol cycle (PI) inhibition and Wnt pathways mimicking properties, which might contribute to its teratogenic effects. In this study the toxic effects of Li2CO3 in chick embryonic cardiomyocyte micromass system (MM) and embryonic stem cell derived cardiomyocyte (ESDC) were evaluated, with possible protective role of myo-inositol. In MM system the Li2CO3 did not alter the toxicity estimation endpoints, whereas in ESDC system the cardiomyocytes contractile activity stopped at 1500 µM and above with significant increase in total cellular protein contents. In ESDC system when myo-inositol was added along with Li2CO3 to continue PI cycle, the contractile activity was recovered with decreased protein content. The lithium toxic effects depend on the role of PI cycle at particular stage of cardiogenesis, while relation between myo-inositol and reduced cellular protein contents remains unknown.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Teratogênicos
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Carbonato de Lítio
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Antimaníacos
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Miócitos Cardíacos
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Células-Tronco Embrionárias
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Inositol
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article