Epigallocatechin-3-O-(3-O-methyl)-gallate-induced differentiation of human keratinocytes involves klotho-mediated regulation of protein kinase-cAMP responsive element-binding protein signaling.
Int J Mol Sci
; 15(4): 5749-61, 2014 Apr 04.
Article
em En
| MEDLINE
| ID: mdl-24714085
ABSTRACT
(-)-Epigallocatechin-3-O-gallate (EGCG) has long been known as a potent inducer of keratinocyte differentiation. Although its molecular mechanisms have been extensively studied, its actions on human skin remain to be elucidated. In this study, we demonstrated that methylated EGCG and EGCG increase the expression of klotho, and that klotho functions as a downstream target of EGCG and methylated EGCG in keratinocyte differentiation. We demonstrated that methylated EGCG3 and EGCG induce morphological changes in normal human epidermal keratinocytes (NHEKs) that are related to up-regulation of klotho expression. We also demonstrated that a klotho-induced keratinocyte differentiation marker in NHEKs is inhibited by H-89, a protein kinase (PKA) inhibitor. These results suggest that methylated EGCG and EGCG may function as inducers of keratinocyte differentiation via transcriptional regulation of the klotho protein.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Queratinócitos
/
Diferenciação Celular
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Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico
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Ácido Gálico
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Glucuronidase
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article