mFISH analysis of chromosome aberrations in workers occupationally exposed to mixed radiation.
Radiat Environ Biophys
; 53(2): 347-54, 2014 May.
Article
em En
| MEDLINE
| ID: mdl-24714826
ABSTRACT
We performed a study on the presence of chromosome aberrations in a cohort of plutonium workers of the Mayak production association (PA) with a mean age of 73.3 ± 7.2 years to see whether by multi-color fluorescence in situ hybridization (mFISH) translocation analysis can discriminate individuals who underwent occupational exposure with internal and/or external exposure to ionizing radiation 40 years ago. All Mayak PA workers were occupationally exposed to chronic internal alpha-radiation due to incorporated plutonium-239 and/or to external gamma-rays. First, we obtained the translocation yield in control individuals by mFISH to chromosome spreads of age-matched individuals and obtained background values that are similar to previously published values of an international study (Sigurdson et al. in Mutat Res 652112-121, 2008). Workers who had absorbed a total dose of >0.5 Gy external gamma-rays to the red bone marrow (RBM) displayed a significantly higher frequency of stable chromosome aberrations relative to a group of workers exposed to <0.5 Gy gamma-rays total absorbed RBM dose. Thus, the translocation frequency may be considered to be a biological marker of external radiation exposure even years after the exposure. In a group of workers who were internally exposed and had incorporated plutonium-239 at a body burden >1.48 kBq, mFISH revealed a considerable number of cells with complex chromosomal rearrangements. Linear associations were observed for translocation yield with the absorbed RBM dose from external gamma-rays as well as for complex chromosomal rearrangements with the plutonium-239 body burden.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Radiometria
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Exposição Ocupacional
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Aberrações Cromossômicas
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Hibridização in Situ Fluorescente
Limite:
Adult
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Female
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Humans
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Male
País como assunto:
Asia
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Europa
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article