Development of a PCR test system for specific detection of Salmonella Paratyphi B in foods.
FEMS Microbiol Lett
; 355(1): 83-9, 2014 Jun.
Article
em En
| MEDLINE
| ID: mdl-24725227
ABSTRACT
Salmonella enterica serotype Paratyphi B is a globally distributed human-specific pathogen causing paratyphoid fever. The aim of this study was to develop a rapid and reliable polymerase chain reaction (PCR) assay for its detection in food. The SPAB_01124 gene was found to be unique to S. Paratyphi B using comparative genomics. Primers for fragments of the SPAB_01124 gene and the Salmonella-specific invA gene were used in combination to establish a multiplex PCR assay that showed 100% specificity across 45 Salmonella strains (representing 34 serotypes) and 18 non-Salmonella strains. The detection limit was 2.2 CFU mL(-1) of S. Paratyphi B after 12-h enrichment in pure culture. It was shown that co-culture with S. Typhimurium or Escherichia coli up to concentrations of 3.6 × 10(5) CFU and 3.3 × 10(4) CFU, respectively, did not interfere with PCR detection of S. Paratyphi B. In artificially contaminated milk, the assay could detect as few as 62 CFU mL(-1) after 8 h of enrichment. In conclusion, comparative genomics was found to be an efficient approach to the mining of pathogen-specific target genes, and the PCR assay that was developed from this provided a rapid, specific, and sensitive method for detection of S. Paratyphi B.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Salmonella paratyphi B
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Reação em Cadeia da Polimerase Multiplex
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Microbiologia de Alimentos
Tipo de estudo:
Diagnostic_studies
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Evaluation_studies
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article