Whole exome sequencing to estimate alloreactivity potential between donors and recipients in stem cell transplantation.
Br J Haematol
; 166(4): 566-70, 2014 Aug.
Article
em En
| MEDLINE
| ID: mdl-24749631
ABSTRACT
Whole exome sequencing (WES) was performed on stem cell transplant donor-recipient (D-R) pairs to determine the extent of potential antigenic variation at a molecular level. In a small cohort of D-R pairs, a high frequency of sequence variation was observed between the donor and recipient exomes independent of human leucocyte antigen (HLA) matching. Nonsynonymous, nonconservative single nucleotide polymorphisms were approximately twice as frequent in HLA-matched unrelated, compared with related D-R pairs. When mapped to individual chromosomes, these polymorphic nucleotides were uniformly distributed across the entire exome. In conclusion, WES reveals extensive nucleotide sequence variation in the exomes of HLA-matched donors and recipients.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Polimorfismo de Nucleotídeo Único
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Tolerância ao Transplante
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Transplante de Células-Tronco
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Exoma
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article