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Generation of iPS Cells from Human Hair Follice Dermal Papilla Cells.
Muchkaeva, I A; Dashinimaev, E B; Artyuhov, A S; Myagkova, E P; Vorotelyak, E A; Yegorov, Y Y; Vishnyakova, K S; Kravchenko, I E; Chumakov, P M; Terskikh, V V; Vasiliev, A V.
Afiliação
  • Muchkaeva IA; Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Vavilova str., 26, 117808, Moscow, Russia ; Pirogov Russian National Research Medical University, Ostrovitianov str., 1, 117997, Moscow, Russia.
  • Dashinimaev EB; Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Vavilova str., 26, 117808, Moscow, Russia ; Pirogov Russian National Research Medical University, Ostrovitianov str., 1, 117997, Moscow, Russia.
  • Artyuhov AS; Pirogov Russian National Research Medical University, Ostrovitianov str., 1, 117997, Moscow, Russia.
  • Myagkova EP; Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Vavilova str., 26, 117808, Moscow, Russia ; Pirogov Russian National Research Medical University, Ostrovitianov str., 1, 117997, Moscow, Russia.
  • Vorotelyak EA; Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Vavilova str., 26, 117808, Moscow, Russia ; Pirogov Russian National Research Medical University, Ostrovitianov str., 1, 117997, Moscow, Russia.
  • Yegorov YY; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vaviolva str., 32, 119991, Moscow, Russia.
  • Vishnyakova KS; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vaviolva str., 32, 119991, Moscow, Russia.
  • Kravchenko IE; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vaviolva str., 32, 119991, Moscow, Russia ; Shemyakin and Ovchinnikov Instituse of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklay str., 16/10, 117997, Moscow, Russia.
  • Chumakov PM; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vaviolva str., 32, 119991, Moscow, Russia ; Shemyakin and Ovchinnikov Instituse of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklay str., 16/10, 117997, Moscow, Russia.
  • Terskikh VV; Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Vavilova str., 26, 117808, Moscow, Russia.
  • Vasiliev AV; Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Vavilova str., 26, 117808, Moscow, Russia.
Acta Naturae ; 6(1): 45-53, 2014 Jan.
Article em En | MEDLINE | ID: mdl-24772326
ABSTRACT
Dermal papilla (DP) cells are unique regional stem cells of the skin that induce formation of a hair follicle and its regeneration cycle. DP are multipotent stem cells; therefore we supposed that the efficiency of DPC reprogramming could exceed that of dermal fibroblasts reprogramming. We generated induced pluripotent stem cells from human DP cells using lentiviral transfection with Oct4, Sox2, Klf4, and c-Myc, and cultivation of cells both in a medium supplemented with valproic acid and at a physiological level of oxygen (5%). The efficiency of DP cells reprogramming was ~0.03%, while the efficiency of dermal fibroblast reprogramming under the same conditions was ~0.01%. Therefore, we demonstrated the suitability of DP cells as an alternative source of iPS cells.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2014 Tipo de documento: Article
Texto completo
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2014 Tipo de documento: Article