[Optimization of Scutellaria baicalensis extraction process by orthogonal experiment combined with pharmacodynamic index].
Zhongguo Zhong Yao Za Zhi
; 38(24): 4314-8, 2013 Dec.
Article
em Zh
| MEDLINE
| ID: mdl-24791537
ABSTRACT
To optimize the Scutellaria baicalensis extraction process, the filter paper method and the bacteriostatic ratio method were adopted to determine the in vitro bacteriostatic efficacy of water extracts and 60% alcohol extracts from S. baicalensis. The quantitative analysis of multi-components by single-marker (QAMS) was used to determined the contents of four active components, baicalin, wogonoside, baicalein and wogonin. In addition, with the bacteriostatic ratio and the overall desirability of the contents of four active components as indexes, the orthogonal experiment was adopted to detect the effect of water addition, extraction frequency and extraction time. The optimal extraction process was to add 12 times of water for the first time, 10 times of water for the second time, extract for 2 time, 2 h for each time. This optimization process is stable and feasible, with a higher bacteriostatic ratio in extracts.
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Base de dados:
MEDLINE
Assunto principal:
Medicamentos de Ervas Chinesas
/
Scutellaria baicalensis
/
Fracionamento Químico
/
Antibacterianos
Idioma:
Zh
Ano de publicação:
2013
Tipo de documento:
Article