Orientation of llama antibodies strongly increases sensitivity of biosensors.
Biosens Bioelectron
; 60: 130-6, 2014 Oct 15.
Article
em En
| MEDLINE
| ID: mdl-24793095
ABSTRACT
Sensitivity of biosensors depends on the orientation of bio-receptors on the sensor surface. The objective of this study was to organize bio-receptors on surfaces in a way that their analyte binding site is exposed to the analyte solution. VHH proteins recognizing foot-and-mouth disease virus (FMDV) were used for making biosensors, and azides were introduced in the VHH to function as bioorthogonal reactive groups. The importance of the orientation of bio-receptors was addressed by comparing sensors with randomly oriented VHH (with multiple exposed azide groups) to sensors with uniformly oriented VHH (with only a single azide group). A surface plasmon resonance (SPR) chip exposing cyclooctyne was reacted to azide functionalized VHH domains, using click chemistry. Comparison between randomly and uniformly oriented bio-receptors showed up to 800-fold increase in biosensor sensitivity. This technique may increase the containment of infectious diseases such as FMDV as its strongly enhanced sensitivity may facilitate early diagnostics.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Camelídeos Americanos
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Imunoensaio
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Técnicas Biossensoriais
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Ressonância de Plasmônio de Superfície
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Vírus da Febre Aftosa
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Anticorpos Antivirais
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article