DNA vaccination by intradermal electroporation induces long-lasting immune responses in rhesus macaques.
J Med Primatol
; 43(5): 329-40, 2014 Oct.
Article
em En
| MEDLINE
| ID: mdl-24810337
ABSTRACT
BACKGROUND:
A desirable HIV vaccine should induce protective long-lasting humoral and cellular immune responses.METHODS:
Macaques were immunized by env DNA, selected from a panel of recently transmitted SIVmac251 Env using intradermal electroporation as vaccine delivery method and magnitude, breadth and longevity of humoral and cellular immune responses.RESULTS:
The macaques developed high, long-lasting humoral immune responses with neutralizing capacity against homologous and heterologous Env. The avidity of the antibody responses was also preserved over 1-year follow-up. Analysis of cellular immune responses demonstrated induction of Env-specific memory T cells harboring granzyme B, albeit their overall levels were low. Similar to the humoral responses, the cellular immunity was persistent over the ~1-year follow-up.CONCLUSION:
These data show that vaccination by this intradermal DNA delivery regimen is able to induce potent and durable immune responses in macaques.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Injeções Intradérmicas
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Síndrome de Imunodeficiência Adquirida dos Símios
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Vírus da Imunodeficiência Símia
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Vacinação
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Eletroporação
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Vacinas contra a SAIDS
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Macaca mulatta
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article