Immunotherapy reduces CD40L expression and modifies cytokine production in the CD4 cells of pollen allergy patients.
J Investig Allergol Clin Immunol
; 24(2): 98-105, 2014.
Article
em En
| MEDLINE
| ID: mdl-24834772
ABSTRACT
BACKGROUND:
Allergen-specific immunotherapy (SIT) is the only intervention for IgE-mediated respiratory disorders.OBJECTIVE:
The aim of the study was to investigate the immunological modifications induced by SIT in patients allergic to olive and/or grass pollen by attempting to establish an association between these modifications and clinical improvements.METHODS:
We studied 29 patients who were allergic to olive and/or grass pollen. Patients were randomized to 2 groups an active treatment group, comprising 19 allergic patients who received SIT, and a control group, formed by 10 allergic patients who received pharmacological treatment for their allergic symptoms but not immunotherapy. We used flow cytometry to analyze intracellular expression of the cytokines IL-4, IFN-gamma, IL-10, and TGF-beta1 in CD4+ T cells, as well as expression of Foxp3, the costimulatory CTLA-4 molecule, and the non-costimulatory CD40L molecule. To assess clinical changes, patients recorded their medication consumption, symptoms, and the limitation of daily activities using diary cards and quality of life questionnaires.RESULTS:
Six months after initiation of SIT, we recorded a reduction in cell surface CD40L expression in the CD4+ T-cell population and a shift in the cytokine production profile (decrease in IL-4-producing CD4+ T cells and increase in IFN-gamma, IL-10, and TGF-beta1). These changes persisted after 12 months. Simultaneously, a clinical improvement was observed.CONCLUSIONS:
SIT-induced clinical improvement is the result of immunological modifications such as a reduction in CD40L expression on CD4 cells and alteration in the cytokine production profile.
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Base de dados:
MEDLINE
Assunto principal:
Linfócitos T CD4-Positivos
/
Rinite Alérgica Sazonal
/
Citocinas
/
Dessensibilização Imunológica
/
Ligante de CD40
Tipo de estudo:
Clinical_trials
Limite:
Adolescent
/
Adult
/
Child
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article