Gambogic acid induces growth inhibition and differentiation via upregulation of p21waf1/cip1 expression in acute myeloid leukemia cells.
J Asian Nat Prod Res
; 16(10): 1000-8, 2014 Oct.
Article
em En
| MEDLINE
| ID: mdl-24835506
ABSTRACT
Gambogic acid (GA) is the major active ingredient of gamboges, a brownish to orange resin product from Garcinia hanburyi tree in Southeast Asia. This compound exhibits anti-cancer effect on solid tumors. In this study, we investigated the effects of GA on the growth and differentiation of acute myeloid leukemia cells by growth-inhibition detection, morphological changes observation, nitroblue tetrazolium reduction, and the expression of the relative cell-surface differentiation markers. The results showed that GA could inhibit cell growth and promote differentiation in U937 and HL-60 cells. In addition, GA upregulated the expression of p21waf1/cip1 in the two cell lines. Finally, downregulating the p21waf1/cip1 expression with small interfering RNA partially blocked GA-induced cell growth inhibition and differentiation. These results of this study revealed that GA may be used as one of the investigational drugs for acute myeloid leukemia.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Garcinia
/
Xantonas
/
Antineoplásicos Fitogênicos
Limite:
Humans
País como assunto:
Asia
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article