Construction of a system for single-cell transgene induction in Caenorhabditis elegans using a pulsed infrared laser.
Methods
; 68(3): 431-6, 2014 Aug 01.
Article
em En
| MEDLINE
| ID: mdl-24835576
ABSTRACT
The spatial and temporal control of transgene expression is an important tool in Caenorhabditis elegans biology. We previously described a method for evoking gene expression in arbitrary cells by using a focused pulsed infrared laser to induce a heat shock response (Churgin et al., 2013). Here we describe detailed methods for building and testing a system for performing single-cell heat shock. Steps include setting up the laser and associated components, coupling the laser beam to a microscope, and testing heat shock protocols. All steps can be carried out using readily available off-the-shelf components.
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MEDLINE
Assunto principal:
Caenorhabditis elegans
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Regulação da Expressão Gênica no Desenvolvimento
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Resposta ao Choque Térmico
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Análise de Célula Única
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article