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A causal relation between bioluminescence and oxygen to quantify the cell niche.
Lambrechts, Dennis; Roeffaers, Maarten; Goossens, Karel; Hofkens, Johan; Vande Velde, Greetje; Van de Putte, Tom; Schrooten, Jan; Van Oosterwyck, Hans.
Afiliação
  • Lambrechts D; Department of Metallurgy and Materials Engineering, KU Leuven, Leuven, Belgium; Prometheus, Division of Skeletal Tissue Engineering Leuven, KU Leuven, Leuven, Belgium.
  • Roeffaers M; Center for Surface Chemistry and Catalysis, KU Leuven, Leuven, Belgium.
  • Goossens K; Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas, United States of America.
  • Hofkens J; Molecular Imaging and Photonics, KU Leuven, Leuven, Belgium.
  • Van de Putte T; TiGenix NV, Leuven, Belgium.
  • Schrooten J; Department of Metallurgy and Materials Engineering, KU Leuven, Leuven, Belgium; Prometheus, Division of Skeletal Tissue Engineering Leuven, KU Leuven, Leuven, Belgium.
  • Van Oosterwyck H; Prometheus, Division of Skeletal Tissue Engineering Leuven, KU Leuven, Leuven, Belgium; Biomechanics Section, KU Leuven, Leuven, Belgium.
PLoS One ; 9(5): e97572, 2014.
Article em En | MEDLINE | ID: mdl-24840204
ABSTRACT
Bioluminescence imaging assays have become a widely integrated technique to quantify effectiveness of cell-based therapies by monitoring fate and survival of transplanted cells. To date these assays are still largely qualitative and often erroneous due to the complexity and dynamics of local micro-environments (niches) in which the cells reside. Here, we report, using a combined experimental and computational approach, on oxygen that besides being a critical niche component responsible for cellular energy metabolism and cell-fate commitment, also serves a primary role in regulating bioluminescent light kinetics. We demonstrate the potential of an oxygen dependent Michaelis-Menten relation in quantifying intrinsic bioluminescence intensities by resolving cell-associated oxygen gradients from bioluminescent light that is emitted from three-dimensional (3D) cell-seeded hydrogels. Furthermore, the experimental and computational data indicate a strong causal relation of oxygen concentration with emitted bioluminescence intensities. Altogether our approach demonstrates the importance of oxygen to evolve towards quantitative bioluminescence and holds great potential for future microscale measurement of oxygen tension in an easily accessible manner.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Oxigênio Tipo de estudo: Qualitative_research Limite: Humans Idioma: En Ano de publicação: 2014 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Oxigênio Tipo de estudo: Qualitative_research Limite: Humans Idioma: En Ano de publicação: 2014 Tipo de documento: Article