Ingested (oral) tocilizumab inhibits EAE.
Cytokine
; 68(2): 86-93, 2014 Aug.
Article
em En
| MEDLINE
| ID: mdl-24845797
ABSTRACT
BACKGROUND:
Blocking the activity of IL-6 can inhibit autoimmune diseases such as rheumatoid arthritis and Crohn's disease.OBJECTIVE:
We examined whether an antibody against IL-6, tocilizumab (TCZ) (Actemra®), used clinically in rheumatoid arthritis (RA) would have similar anti-inflammatory effects in EAE after oral administration. DESIGN/METHOD:
B6 mice were immunized with MOG peptide 35-55 and gavaged with control saline or TCZ during ongoing disease. Splenocytes, CD4(+) T cells or macrophages/monocyte lineage cells (CD11b(+)) from control fed or TCZ fed mice were adoptively transferred into active MOG peptide 35-55 immunized recipient mice during ongoing disease. Actively fed and recipient mice were examined for disease inhibition, inflammation, and cytokine responses.RESULTS:
Ingested (oral) TCZ inhibited ongoing disease and decreased inflammation. Adoptively transferred cells from TCZ fed donors protected against actively induced disease and decreased inflammation. There was a decrease in IL-6 in actively treated spleen, decrease in TNF-α, Th1-like cytokine IL-12 and increase in Th2-like cytokine IL-10 in active fed and adoptively treated recipients.CONCLUSIONS:
Ingested (orally administered) TCZ can inhibit disease, CNS inflammation, decrease pro-inflammatory Th1-like cytokines and increase Th2-like anti-inflammatory cytokines.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Encefalomielite Autoimune Experimental
/
Anticorpos Monoclonais Humanizados
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article