The effect of epigallocatechin gallate on hepatocytes isolated from normal and partially hepatectomized rats.
Can J Physiol Pharmacol
; 92(6): 512-7, 2014 Jun.
Article
em En
| MEDLINE
| ID: mdl-24853265
ABSTRACT
Epigallocatechin gallate (EGCG) is an antioxidant found in green tea. In this study, male Wistar rats were subjected either to partial hepatectomy (PHx), or a sham operation (LAP). Twenty-four hours after surgery, hepatocytes were isolated and treated with various concentrations of EGCG for up to 72 h. We then measured markers of cell viability, oxidative stress, DNA synthesis, and caspase activity. Morphological criteria, cell viability tests, and albumin synthesis revealed toxicity starting at 10 µmol/L. DNA synthesis was higher in hepatocytes isolated from rats after PHx and inhibited by EGCG. Furthermore, EGCG increased the activity of caspases 3 and 7, seen more in hepatocytes from PHx rats. In conclusion, EGCG at a concentration of 10 µmol/L was toxic for hepatocytes isolated from both PHx and LAP rats.
Palavras-chave
antioxidants; antioxydants; apoptose; apoptosis; catechin; catéchine; culture cellulaire primaire; epigallocatechin gallate; hepatectomy; hepatocytes; hépatectomie; hépatocytes; le gallate d'épigallocatéchine; liver regeneration; oxidative stress; primary cell culture; régénération du foie; stress oxydatif
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Catequina
/
Hepatócitos
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Hepatectomia
/
Antioxidantes
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article