Mechanical feedback through E-cadherin promotes direction sensing during collective cell migration.
Cell
; 157(5): 1146-59, 2014 May 22.
Article
em En
| MEDLINE
| ID: mdl-24855950
ABSTRACT
E-cadherin is a major homophilic cell-cell adhesion molecule that inhibits motility of individual cells on matrix. However, its contribution to migration of cells through cell-rich tissues is less clear. We developed an in vivo sensor of mechanical tension across E-cadherin molecules, which we combined with cell-type-specific RNAi, photoactivatable Rac, and morphodynamic profiling, to interrogate how E-cadherin contributes to collective migration of cells between other cells. Using the Drosophila ovary as a model, we found that adhesion between border cells and their substrate, the nurse cells, functions in a positive feedback loop with Rac and actin assembly to stabilize forward-directed protrusion and directionally persistent movement. Adhesion between individual border cells communicates direction from the lead cell to the followers. Adhesion between motile cells and polar cells holds the cluster together and polarizes each individual cell. Thus, E-cadherin is an integral component of the guidance mechanisms that orchestrate collective chemotaxis in vivo.
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Base de dados:
MEDLINE
Assunto principal:
Caderinas
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Movimento Celular
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Proteínas de Drosophila
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Drosophila melanogaster
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article