Structural basis for the inhibition of host protein ubiquitination by Shigella effector kinase OspG.
Structure
; 22(6): 878-88, 2014 Jun 10.
Article
em En
| MEDLINE
| ID: mdl-24856362
ABSTRACT
Shigella invasion of its human host is assisted by T3SS-delivered effector proteins. The OspG effector kinase binds ubiquitin and ubiquitin-loaded E2-conjugating enzymes, including UbcH5b and UbcH7, and attenuates the host innate immune NF-kB signaling. We present the structure of OspG bound to the UbcH7â¼Ub conjugate. OspG has a minimal kinase fold lacking the activation loop of regulatory kinases. UbcH7â¼Ub binds OspG at sites remote from the kinase active site, yet increases its kinase activity. The ubiquitin is positioned in the "open" conformation with respect to UbcH7 using its I44 patch to interact with the C terminus of OspG. UbcH7 binds to OspG using two conserved loops essential for E3 ligase recruitment. The interaction of the UbcH7â¼Ub with OspG is remarkably similar to the interaction of an E2â¼Ub with a HECT E3 ligase. OspG interferes with the interaction of UbcH7 with the E3 parkin and inhibits the activity of the E3.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Shigella flexneri
/
Proteínas de Bactérias
/
Interações Hospedeiro-Patógeno
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article