ß-lactoglobulin as a vector for ß-carotene food fortification.
J Agric Food Chem
; 62(25): 5916-24, 2014 Jun 25.
Article
em En
| MEDLINE
| ID: mdl-24857546
ABSTRACT
Food fortification is a strategy to overcome vitamin A deficiency in developing countries. Our aim was to investigate the involvement of the bovine milk protein ß-lactoglobulin (ß-Lg), a potential retinoid carrier, in vitamin A absorption. In vivo experiments were conducted by force-feeding mice with retinol or ß-carotene associated with either ß-Lg or oil-in-water emulsion, with subsequent determination of both vitamin A intestinal mucosa and plasma contents. Caco-2 cells were then used to investigate the mechanisms of vitamin A uptake when delivered by either ß-Lg or mixed micelles. We showed that ß-Lg was as efficient as emulsion to promote ß-carotene, but not retinol, absorption in mice. Similar results were obtained in vitro. Interestingly, an inhibitor of the Scavenger Receptor Class B Type I significantly decreased the uptake of micellar ß-carotene but not that of ß-carotene bound to ß-Lg. Overall, we showed that ß-Lg would be a good vector for ß-carotene food fortification.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Deficiência de Vitamina A
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Portadores de Fármacos
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Alimentos Fortificados
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Beta Caroteno
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Lactoglobulinas
Limite:
Animals
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Humans
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Male
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article