Clostridium bornimense sp. nov., isolated from a mesophilic, two-phase, laboratory-scale biogas reactor.
Int J Syst Evol Microbiol
; 64(Pt 8): 2792-2797, 2014 Aug.
Article
em En
| MEDLINE
| ID: mdl-24860110
ABSTRACT
A novel anaerobic, mesophilic, hydrogen-producing bacterium, designated strain M2/40(T), was isolated from a mesophilic, two-phase, laboratory-scale biogas reactor fed continuously with maize silage supplemented with 5% wheat straw. 16S rRNA gene sequence comparison revealed an affiliation to the genus Clostridium sensu stricto (cluster I of the clostridia), with Clostridium cellulovorans as the closest characterized species, showing 93.8% sequence similarity to the type strain. Cells of strain M2/40(T) were rods to elongated filamentous rods that showed variable Gram staining. Optimal growth occurred at 35 °C and at pH 7. Grown on glucose, the main fermentation products were H2, CO2, formate, lactate and propionate. The DNA G+C content was 29.6 mol%. The major fatty acids (>10â%) were C(16ââ0), summed feature 10 (C(18ââ1)ω11c/ω9t/ω6t and/or unknown ECL 17.834) and C(18ââ1)ω11c dimethylacetal. Based on phenotypic, chemotaxonomic and phylogenetic differences, strain M2/40(T) represents a novel species within the genus Clostridium, for which we propose the name Clostridium bornimense sp. nov. The type strain is M2/40(T) (â=âDSM 25664(T)â=âCECT 8097(T)).
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Filogenia
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Silagem
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Clostridium
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Reatores Biológicos
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Biocombustíveis
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article