TCR Microclusters pre-exist and contain molecules necessary for TCR signal transduction.
J Immunol
; 193(1): 56-67, 2014 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-24860189
TCR-dependent signaling events have been observed to occur in TCR microclusters. We found that some TCR microclusters are present in unstimulated murine T cells, indicating that the mechanisms leading to microcluster formation do not require ligand binding. These pre-existing microclusters increase in absolute number following engagement by low-potency ligands. This increase is accompanied by an increase in cell spreading, with the result that the density of TCR microclusters on the surface of the T cell is not a strong function of ligand potency. In characterizing their composition, we observed a constant number of TCRs in a microcluster, constitutive exclusion of the phosphatase CD45, and preassociation with the signaling adapters linker for activation of T cells and growth factor receptor-bound protein 2. The existence of TCR microclusters prior to ligand binding in a state that is conducive for the initiation of downstream signaling could explain, in part, the rapid kinetics with which TCR signal transduction occurs.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Receptores de Antígenos de Linfócitos T
/
Linfócitos T
/
Transdução de Sinais
/
Antígenos Comuns de Leucócito
/
Microdomínios da Membrana
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article