Theoretical and experimental study of the role of cell-cell dipole interaction in dielectrophoretic devices: application to polynomial electrodes.
Biomed Eng Online
; 13: 71, 2014 Jun 05.
Article
em En
| MEDLINE
| ID: mdl-24903282
ABSTRACT
BACKGROUND:
We aimed to investigate the effect of cell-cell dipole interactions in the equilibrium distributions in dielectrophoretic devices.METHODS:
We used a three dimensional coupled Monte Carlo-Poisson method to theoretically study the final distribution of a system of uncharged polarizable particles suspended in a static liquid medium under the action of an oscillating non-uniform electric field generated by polynomial electrodes. The simulated distributions have been compared with experimental ones observed in the case of MDA-MB-231 cells in the same operating conditions.RESULTS:
The real and simulated distributions are consistent. In both cases the cells distribution near the electrodes is dominated by cell-cell dipole interactions which generate long chains.CONCLUSIONS:
The agreement between real and simulated cells' distributions demonstrate the method's reliability. The distribution are dominated by cell-cell dipole interactions even at low density regimes (105 cell/ml). An improved estimate for the density threshold governing the interaction free regime is suggested.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Comunicação Celular
/
Eletroforese
Tipo de estudo:
Health_economic_evaluation
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article