Analysis of the cell surface expression of cytokine receptors using the surface protein biotinylation method.
Methods Mol Biol
; 1172: 185-92, 2014.
Article
em En
| MEDLINE
| ID: mdl-24908305
ABSTRACT
Cytokines are pleiotropic, low-molecular-weight proteins that regulate the immune responses to infection and inflammation. They stimulate the immune responses by binding to cytokine receptors on the cell plasma membrane. Thus, knowledge of the expression level of particular cytokine receptors on cell surface is crucial for understanding the cytokine function and regulation. One of the techniques to explore the membrane embedded cytokine receptors is cell surface biotinylation. Biotinylated surface proteins can be rapidly purified through the strong interaction between biotin and streptavidin. Here, we describe the procedure for surface biotinylation and purification of biotinylated cytokine receptors for further downstream analysis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sefarose
/
Coloração e Rotulagem
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Succinimidas
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Proteínas de Bactérias
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Biotina
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Receptores de Citocinas
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article