Engineering the specificity of trehalose phosphorylase as a general strategy for the production of glycosyl phosphates.
Chem Commun (Camb)
; 50(58): 7834-6, 2014 Jul 25.
Article
em En
| MEDLINE
| ID: mdl-24909572
ABSTRACT
A two-step process is reported for the anomeric phosphorylation of galactose, using trehalose phosphorylase as biocatalyst. The monosaccharide enters this process as acceptor but can subsequently be released from the donor side, thanks to the non-reducing nature of the disaccharide intermediate. A key development was the creation of an optimized enzyme variant that displays a strict specificity (99%) for ß-galactose 1-phosphate as product.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fosfatos
/
Galactose
/
Glucosiltransferases
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article