From confluent human iPS cells to self-forming neural retina and retinal pigmented epithelium.
Proc Natl Acad Sci U S A
; 111(23): 8518-23, 2014 Jun 10.
Article
em En
| MEDLINE
| ID: mdl-24912154
ABSTRACT
Progress in retinal-cell therapy derived from human pluripotent stem cells currently faces technical challenges that require the development of easy and standardized protocols. Here, we developed a simple retinal differentiation method, based on confluent human induced pluripotent stem cells (hiPSC), bypassing embryoid body formation and the use of exogenous molecules, coating, or Matrigel. In 2 wk, we generated both retinal pigmented epithelial cells and self-forming neural retina (NR)-like structures containing retinal progenitor cells (RPCs). We report sequential differentiation from RPCs to the seven neuroretinal cell types in maturated NR-like structures as floating cultures, thereby revealing the multipotency of RPCs generated from integration-free hiPSCs. Furthermore, Notch pathway inhibition boosted the generation of photoreceptor precursor cells, crucial in establishing cell therapy strategies. This innovative process proposed here provides a readily efficient and scalable approach to produce retinal cells for regenerative medicine and for drug-screening purposes, as well as an in vitro model of human retinal development and disease.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Diferenciação Celular
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Neurônios Retinianos
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Epitélio Pigmentado da Retina
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Células-Tronco Pluripotentes Induzidas
Tipo de estudo:
Guideline
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Prognostic_studies
Limite:
Adult
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Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article