Your browser doesn't support javascript.
loading
Cisplatin-induced renal injury is independently mediated by OCT2 and p53.
Sprowl, Jason A; Lancaster, Cynthia S; Pabla, Navjotsingh; Hermann, Edwin; Kosloske, Ashley M; Gibson, Alice A; Li, Lie; Zeeh, Dorothea; Schlatter, Eberhard; Janke, Laura J; Ciarimboli, Giuliano; Sparreboom, Alex.
Afiliação
  • Sprowl JA; Departments of Pharmaceutical Sciences and.
  • Lancaster CS; Departments of Pharmaceutical Sciences and.
  • Pabla N; Departments of Pharmaceutical Sciences and.
  • Hermann E; Klinik für Urologie and.
  • Kosloske AM; Departments of Pharmaceutical Sciences and.
  • Gibson AA; Departments of Pharmaceutical Sciences and.
  • Li L; Departments of Pharmaceutical Sciences and.
  • Zeeh D; Medizinische Klinik D, Experimentelle Nephrologie, Universitätsklinikum Münster, Münster, Germany.
  • Schlatter E; Medizinische Klinik D, Experimentelle Nephrologie, Universitätsklinikum Münster, Münster, Germany.
  • Janke LJ; Pathology, St. Jude Children's Research Hospital, Memphis, Tennessee; and.
  • Ciarimboli G; Medizinische Klinik D, Experimentelle Nephrologie, Universitätsklinikum Münster, Münster, Germany.
  • Sparreboom A; Departments of Pharmaceutical Sciences and alex.sparreboom@stjude.org.
Clin Cancer Res ; 20(15): 4026-35, 2014 Aug 01.
Article em En | MEDLINE | ID: mdl-24916697
ABSTRACT

PURPOSE:

Tubular secretion of cisplatin is abolished in mice deficient for the organic cation transporters Oct1 and Oct2 (Oct1/2(-/-)mice), and these animals are protected from severe cisplatin-induced kidney damage. Since tubular necrosis is not completely absent in Oct1/2(-/-)mice, we hypothesized that alternate pathways are involved in the observed injury. EXPERIMENTAL

DESIGN:

Studies were done in wild-type, Oct1/2(-/-), or p53-deficient animals, all on an FVB background, receiving cisplatin intraperitoneally at 15 mg/kg. Cisplatin metabolites were analyzed using mass spectrometry, and gene expression was assessed using Affymetrix microarrays and RT-PCR arrays.

RESULTS:

KEGG pathway analyses on kidneys from mice exposed to cisplatin revealed that the most significantly altered genes were associated with the p53 signaling network, including Cdnk1a and Mdm2, in both wild-type (P = 2.40 × 10(-11)) and Oct1/2(-/-)mice (P = 1.92 × 10(-8)). This was confirmed by demonstrating that homozygosity for a p53-null allele partially reduced renal tubular damage, whereas loss of p53 in Oct1/2(-/-)mice (p53(-/-)/Oct1/2(-/-)) completely abolished nephrotoxicity. We found that pifithrin-α, an inhibitor of p53-dependent transcriptional activation, inhibits Oct2 and can mimic the lack of nephrotoxicity observed in p53(-/-)/Oct1/2(-/-)mice.

CONCLUSIONS:

These findings indicate that (i) the p53 pathway plays a crucial role in the kidney in response to cisplatin treatment and (ii) clinical exploration of OCT2 inhibitors may not lead to complete nephroprotection unless the p53 pathway is simultaneously antagonized.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteína Supressora de Tumor p53 / Cisplatino / Proteínas de Transporte de Cátions Orgânicos / Fator 1 de Transcrição de Octâmero / Nefropatias / Antineoplásicos Limite: Animals Idioma: En Ano de publicação: 2014 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteína Supressora de Tumor p53 / Cisplatino / Proteínas de Transporte de Cátions Orgânicos / Fator 1 de Transcrição de Octâmero / Nefropatias / Antineoplásicos Limite: Animals Idioma: En Ano de publicação: 2014 Tipo de documento: Article