Lentiviral vector transduction of spermatozoa as a tool for the study of early development.

Chandrashekran, Anil; Isa, Ihsan; Dudhia, Jayesh; Thrasher, Adrian J; Dibb, Nicholas; Casimir, Colin; Readhead, Carol; Winston, Robert.

Afiliação

Chandrashekran A; Department of Surgery and Cancer, Division of Cancer, Imperial College London, Hammersmith Campus, Institute of Reproductive and Developmental Biology (IRDB), Du Cane Road, London W12 0NN, UK.
Isa I; Department of Surgery and Cancer, Division of Cancer, Imperial College London, Hammersmith Campus, Institute of Reproductive and Developmental Biology (IRDB), Du Cane Road, London W12 0NN, UK.
Dudhia J; Department of Clinical Science Services, The Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Herts AL9 7TA, UK.
Thrasher AJ; Molecular Immunology Unit, UCL-Institute of Child Health, 30 Guilford Street, London WC1N 1EH, UK.
Dibb N; Department of Surgery and Cancer, Division of Cancer, Imperial College London, Hammersmith Campus, Institute of Reproductive and Developmental Biology (IRDB), Du Cane Road, London W12 0NN, UK.
Casimir C; Department of Natural Sciences, School of Science & Technology, Middlesex University, The Burroughs, London NW4 4BT, UK.
Readhead C; Biological Imaging Center, California Institute of Technology, Beckman Institute, 139-74, Pasadena, CA 91135, USA.
Winston R; Department of Surgery and Cancer, Division of Cancer, Imperial College London, Hammersmith Campus, Institute of Reproductive and Developmental Biology (IRDB), Du Cane Road, London W12 0NN, UK.

FEBS Open Bio ; 4: 266-75, 2014.

Article em En | MEDLINE | ID: mdl-24918038

Palavras-chave

293T, Human embryonic kidney cells; 7-AAD, 7-Aminoactinomycin D; AZT, azidodeoxythimidine; CMV, Cytomegalovirus promoter; Development; EF-1, Elongation factor 1 alpha promoter; GFP, Green Fluorescent Protein; IVF, in vitro fertilisation; In vitro fertilisation; LTR, Long Terminal Repeat; Lentiviral vectors; PGK, Phosphoglycerate kinase promoter; Spermatozoa; Transduction; Transgenics; UCOE, ubiquitous chromatin opening element promoter; VSV-g, vesicular stomatitis virus

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2014 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2014 Tipo de documento: Article