Upregulation of relaxin receptors in the PDL by biophysical force.
Clin Oral Investig
; 19(3): 657-65, 2015 Apr.
Article
em En
| MEDLINE
| ID: mdl-24994576
ABSTRACT
OBJECTIVES:
We have previously reported that relaxin (Rln) expression from the ovary is upregulated by orthodontic tooth movement. This study was performed to test the hypothesis that Rln family peptides (Rxfps), the G-protein-coupled Rln receptor, is induced in periodontal ligament (PDL) cells to modulate the molecules involved in periodontal tissue remodeling while applying biophysical force. MATERIALS ANDMETHODS:
Rats were implanted with orthodontic appliances to investigate changes to Rxfps in vivo. An in vitro biophysical force analysis was performed to measure the level of Rxfp 1 messenger RNA (mRNA) in primary human PDL cells.RESULTS:
The levels of Rxfp 2 transcription and translation increased in a time-dependent manner during tooth movement. Rxfp 2 was localized in the PDL by immunofluorescence. In vitro analyses revealed that the level of Rxfp 1 mRNA in PDL cells increased significantly with both compression and tension force. The levels of matrix metalloproteinase (MMP)-1, MMP-2, interleukin-6, and vascular endothelial growth factor mRNA, which are important for periodontal tissue remodeling, also changed under force application and Rln treatment.CONCLUSIONS:
PDL cells responded to Rln to modulate effector molecules for periodontal tissue remodeling by upregulating Rxfps expression under a biophysical force. CLINICAL RELEVANCE Rln and Rxfps may serve as a PDL turnover molecule complex to control orthodontic tooth movement.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Ligamento Periodontal
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Técnicas de Movimentação Dentária
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Receptores de Peptídeos
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Receptores Acoplados a Proteínas G
Limite:
Animals
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Female
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Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article