Your browser doesn't support javascript.
loading
SeptiFast for diagnosis of sepsis in severely ill patients from a Brazilian hospital.
Sitnik, Roberta; Marra, Alexandre Rodrigues; Petroni, Roberta Cardoso; Ramos, Ozires Pereira Santos; Martino, Marinês Dalla Valle; Pasternak, Jacyr; Santos, Oscar Fernando Pavão dos; Mangueira, Cristóvão Luis Pitangueira; Pinho, João Renato Rebello.
Afiliação
  • Sitnik R; Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
  • Marra AR; Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
  • Petroni RC; Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
  • Ramos OP; Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
  • Martino MD; Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
  • Pasternak J; Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
  • Santos OF; Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
  • Mangueira CL; Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
  • Pinho JR; Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
Einstein (Sao Paulo) ; 12(2): 191-6, 2014 Apr.
Article em En, Pt | MEDLINE | ID: mdl-25003925
OBJECTIVE: To test and validate a multiplex real-time polymerase chain reaction method for bloodstream infections, as well as to compare the results with conventional blood culture. METHODS: A total of 114 consecutive patients with clinical evidence of sepsis were submitted to blood culture and LightCycler™ SeptiFast tests. RESULTS: More positive specimens (23; 20.2%) were detected using the LightCycler™ SeptiFast than the blood culture (17; 14.9%), with an agreement of 86.8%. Discordant results were seen in four patients positive only to blood culture, ten positive only to LightCycler™ SeptiFast and one to different pathogens found by each test. Infections with microorganisms detected only using blood culture reassured the need to perform both tests. The mean time to results for blood culture was 5 days for negative and 3.5 days for positive results. LightCycler™ SeptiFast results were achieved in less than 8 hours. CONCLUSION: LightCycler™ SeptiFast showed a high potential as a test to be carried out concomitantly with blood culture for sepsis diagnosis in severely ill patients. This test allowed a faster diagnosis of bacterial and fungal infections that helped to reduce hospital stay and to control the use of antibiotics. LightCycler™ SeptiFast can also eventually detect microorganism and infections that are hardly detected by blood culture, especially Candida non-albicans infections.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Bacteriemia / Técnicas de Diagnóstico Molecular Tipo de estudo: Diagnostic_studies / Observational_studies Limite: Female / Humans / Male / Middle aged País como assunto: America do sul / Brasil Idioma: En / Pt Ano de publicação: 2014 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Bacteriemia / Técnicas de Diagnóstico Molecular Tipo de estudo: Diagnostic_studies / Observational_studies Limite: Female / Humans / Male / Middle aged País como assunto: America do sul / Brasil Idioma: En / Pt Ano de publicação: 2014 Tipo de documento: Article