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Determination of the botanical origin of honey by front-face synchronous fluorescence spectroscopy.
Lenhardt, Lea; Zekovic, Ivana; Dramicanin, Tatjana; Dramicanin, Miroslav D; Bro, Rasmus.
Afiliação
  • Lenhardt L; University of Belgrade, Vinca Institute of Nuclear Sciences, PO Box 522, 11001 Belgrade, Serbia.
Appl Spectrosc ; 68(5): 557-63, 2014.
Article em En | MEDLINE | ID: mdl-25014599
Front-face synchronous fluorescence spectroscopy combined with chemometrics is used to classify honey samples according to their botanical origin. Synchronous fluorescence spectra of three monofloral (linden, sunflower, and acacia), polyfloral (meadow mix), and fake (fake acacia and linden) honey types (109 samples) were collected in an excitation range of 240-500 nm for synchronous wavelength intervals of 30-300 nm. Chemometric analysis of the gathered data included principal component analysis and partial least squares discriminant analysis. Mean cross-validated classification errors of 0.2 and 4.8% were found for a model that accounts only for monofloral samples and for a model that includes both the monofloral and polyfloral groups, respectively. The results demonstrate that single synchronous fluorescence spectra of different honeys differ significantly because of their distinct physical and chemical characteristics and provide sufficient data for the clear differentiation among honey groups. The spectra of fake honey samples showed pronounced differences from those of genuine honey, and these samples are easily recognized on the basis of their synchronous fluorescence spectra. The study demonstrated that this method is a valuable and promising technique for honey authentication.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pólen / Espectrometria de Fluorescência / Néctar de Plantas / Mel Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2014 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pólen / Espectrometria de Fluorescência / Néctar de Plantas / Mel Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2014 Tipo de documento: Article