ECM stiffness regulates glial migration in Drosophila and mammalian glioma models.
Development
; 141(16): 3233-42, 2014 Aug.
Article
em En
| MEDLINE
| ID: mdl-25063458
ABSTRACT
Cell migration is an important feature of glial cells. Here, we used the Drosophila eye disc to decipher the molecular network controlling glial migration. We stimulated glial motility by pan-glial PDGF receptor (PVR) activation and identified several genes acting downstream of PVR. Drosophila lox is a non-essential gene encoding a secreted protein that stiffens the extracellular matrix (ECM). Glial-specific knockdown of Integrin results in ECM softening. Moreover, we show that lox expression is regulated by Integrin signaling and vice versa, suggesting that a positive-feedback loop ensures a rigid ECM in the vicinity of migrating cells. The general implication of this model was tested in a mammalian glioma model, where a Lox-specific inhibitor unraveled a clear impact of ECM rigidity in glioma cell migration.
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Base de dados:
MEDLINE
Assunto principal:
Neuroglia
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Proteínas de Drosophila
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Drosophila melanogaster
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Matriz Extracelular
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Olho Composto de Artrópodes
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Proteína-Lisina 6-Oxidase
Limite:
Animals
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Female
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Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article